Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0002p001 | (1) | SRF2015

Sperm preparation technique affects functional motility in human sperm

Pendekanti Venkata , Publicover Stephen J

Human sperm use different behaviours for different aspects of sperm transport and fertilisation. We have investigated the effects of preparation method (density gradient method and direct swim-up) on sperm behaviour and manipulated Ca2+ signalling (the primary regulator of sperm motility) to induce different behaviours and assessed their effects on penetration of human sperm through viscous medium (methylcellulose).Cells prepared by swim-up pe...